DNA
Making Bio

Part:BBa_K4329001:Design

Designed by: Yuxin He   Group: iGEM22_BS_United_China   (2022-09-24)


Overexpression of dethiobiotin synthase and biotin synthase in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 promoter is an efficient promoter for

Lac operator is inhibits protein and enzyme production without the presence of IPTG

T7 terminator is used to stop the transcription.



Source

References